Human scrotum in a relaxed state (left) and a tense state (right)
|Artery||Anterior scrotal artery & Posterior scrotal artery|
|Nerve||Posterior scrotal nerves, Anterior scrotal nerves, genital branch of genitofemoral nerve, perineal branches of posterior femoral cutaneous nerve|
|Lymph||Superficial inguinal lymph nodes|
The scrotum is an anatomical male reproductive structure located caudal to the penis that consists of a suspended dual-chambered sack of skin and smooth muscle. It is present in most terrestrial male mammals. The scrotum contains the external spermatic fascia, testes, epididymis, and ductus deferens. It is a distention of the perineum and carries some abdominal tissues into its cavity including the testicular artery, testicular vein, and pampiniform plexus. The perineal raphe is a small, vertical, slightly raised ridge of scrotal skin under which is found the scrotal septum. It appears as a thin longitudinal line that runs front to back over the entire scrotum. In humans and some other mammals the scrotum becomes covered with pubic hair at puberty. The scrotum will usually tighten during penile erection and when exposed to cold temperatures. One testis is typically lower than the other to avoid compression in the event of an impact.
The scrotum is biologically homologous to the labia majora in females. Although present in most mammals, the external scrotum is absent in streamlined marine mammals, such as whales and seals, as well as in some lineages of land mammals, such as the afrotherians, xenarthrans, and numerous families of bats, rodents, and insectivores.
|Genital branch of genitofemoral nerve||anterolateral|
|Anterior scrotal nerves (from ilioinguinal nerve)||anterior|
|Posterior scrotal nerves (from perineal nerve)||posterior|
|perineal branches of posterior femoral cutaneous nerve||inferior|
|Anterior scrotal artery||originates from the deep external pudendal artery|
|Posterior scrotal artery|
The skin on the scrotum is more highly pigmented compared to the rest of the body. The septum is a connective tissue membrane dividing the scrotum into two cavities. 
The scrotum lymph drains initially into the superficial inguinal lymph nodes, this then drains into the deep inguinal lymph nodes. The deep inguinal lymph nodes drain into the common iliac which ultimately releases lymph into the cisterna chyli.
One testis is typically lower than the other, which is believed to function to avoid compression in the event of impact; in humans, the left testis is typically lower than the right. An alternative view is that testis descent asymmetry evolved to enable more effective cooling of the testicles.
Additional tissues and organs reside inside the scrotum and are described in more detail in the following articles:
Male sex hormones are secreted by the testes later in embryonic life to cause the development of secondary sex organs. The scrotum is developmentally homologous to the labia majora. The raphe does not exist in females. Reproductive organs and tissues develop in females and males begin during the fifth week after fertilization. The gonadal ridge grows behind the peritoneal membrane. By the sixth week, string-like tissues called primary sex cords form within the enlarging gonadal ridge. Externally, a swelling called the genital tubercule appears over the cloacal membrane.
Up until the eighth week after fertilization, the reproductive organs do not appear to be different between the male and female and are called in-differentiated. Testosterone secretion starts during week eight, reaches peak levels during week 13 and eventually declines to very low levels by the end of the second trimester. The testosterone causes the masculinization of the labioscrotal folds into the scrotum. The scrotal raphe is formed when the embryonic, urethral groove closes by week 12.
Though the testes and scrotum form early in embryonic life, sexual maturation begins upon entering puberty. The increased secretion of testosterone causes the darkening of the skin and development of pubic hair on the scrotum.
The scrotum regulates the temperature of the testes and maintains it at 35 degrees Celsius (95 degrees Fahrenheit), i.e. two or three degrees below the body temperature of 37 degrees Celsius (98.6 degrees Fahrenheit). Higher temperatures affect spermatogenesis Temperature control is accomplished by the smooth muscles of the scrotum moving the testicles either closer to or further away from the abdomen dependent upon the ambient temperature. This is accomplished by the cremaster muscle in the abdomen and the dartos fascia (muscular tissue under the skin).
Having the scrotum and testicles situated outside the abdominal cavity may provide additional advantages. The external scrotum is not affected by abdominal pressure. This may prevent the emptying of the testes before the sperm were matured sufficiently for fertilization. Another advantage is it protects the testes from jolts and compressions associated with an active lifestyle. Animals that move at a steady pace - such as elephants, whales, and marsupial moles - have internal testes and no scrotum. Unlike placental mammals, some male marsupials have a scrotum that is anterior to the penis, which is not homologous to the scrotum of placental mammals, although there are several marsupial species without an external scrotum. In humans, the scrotum may provide some friction during intercourse, helping to enhance the activity.
The scrotum and its contents can develop diseases or incur injuries. These include:
The rear-entry position of mating may allow the scrotum to stimulate the clitoris and, in this way, may produce an orgasm ...