RT-QuIC
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RT-QuIC

Real-time quaking-induced conversion (RT-QuIC) is a highly sensitive assay for prion detection. It is "an early, rapid and specific assay for prion diseases". It samples cerebrospinal fluid (CSF) and so it is applicable to scrapie, chronic wasting disease (CWD), bovine spongiform encephalopathy (BSE) and sporadic Creutzfeldt-Jakob disease, amongst others.[1]

The RT-QuIC assay uses as reagents[clarification needed] normally folded prions, fluorescently labelled so that they indicate when they are misfolded; samples suspected of containing misfolded prions are added and misfolded reagents can be detected by thioflavin T visible spectrum fluorescence detection.[2][3][4] The Centers for Disease Control and Prevention includes a positive RT-QuIC result in its diagnostic criteria for the probable diagnosis of sCJD.[5]

See also

References

  1. ^ Atarashi, R.; Sano, K.; Satoh, K.; Nishida, N. (2011). "Real-time quaking-induced conversion: A highly sensitive assay for prion detection". Prion. 5 (3): 150-3. doi:10.4161/pri.5.3.16893. PMC 3226039. PMID 21778820.
  2. ^ Haley NJ, Hoover EA (2015). "Chronic wasting disease of cervids: current knowledge and future perspectives". Annual Review of Animal Biosciences. 3: 305-25. doi:10.1146/annurev-animal-022114-111001. PMID 25387112.
  3. ^ Atarashi R, Satoh K, Sano K, Fuse T, Yamaguchi N, Ishibashi D, Matsubara T, Nakagaki T, Yamanaka H, Shirabe S, Yamada M, Mizusawa H, Kitamoto T, Klug G, McGlade A, Collins SJ, Nishida N (February 2011). "Ultrasensitive human prion detection in cerebrospinal fluid by real-time quaking-induced conversion" (PDF). Nature Medicine. 17 (2): 175-78. doi:10.1038/nm.2294. hdl:10069/25642. PMID 21278748.
  4. ^ Orrú CD, Bongianni M, Tonoli G, Ferrari S, Hughson AG, Groveman BR, Fiorini M, Pocchiari M, Monaco S, Caughey B, Zanusso G (August 2014). "A test for Creutzfeldt-Jakob disease using nasal brushings". The New England Journal of Medicine. 371 (6): 519-29. doi:10.1056/NEJMoa1315200. PMC 4186748. PMID 25099576.
  5. ^ "CDC's Diagnostic Criteria for Creutzfeldt-Jakob Disease (CJD), 2018". Centers for Disease Control and Prevention. September 17, 2018. Retrieved 2018. Adapted from: a) Global surveillance, diagnosis, and therapy of human transmissible spongiform encephalopathies: Report of a WHO consultation, February 1998, Geneva, Switzerland; b) Zerr I, Kallenberg K, Summers DM, et al. Updated clinical diagnostic criteria for sporadic Creutzfeldt-Jakob disease. Brain 2009, 132; 2659-2668; and c) National CJD Research & Surveillance Unit. Protocol: Surveillance of CJD in the UK. Item c accessed 15 Aug 2018.

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RT-QuIC
 



 



 
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